In Vitro Cytotoxic Effects of Secondary Metabolites Present in Sarcopoterium Spinosum L.

Sarcopoterium spinosum (L.) is a medicinal plant traditionally used for the treatment of various diseases including cancer in Near- and Middle East. The fractions constituents ethanol extract S. were screened vitro cytotoxic activities on Jurkat (acute T-lymphoblastic leukemia), HeLa (cervical adenocarcinoma), MCF-7 (mammary gland Caco-2 (human colorectal MDA-MB-231 adenocarcinoma) cell lines using MTT (3-(dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. was subsequently re-extracted with ethyl acetate its sub-fraction obtained by column chromatography three compounds (stachydrine, benzalkonium chloride rutine) first time identified nuclear magnetic resonance (NMR) analyses. most active subfraction showed activity against HeLa, MCF-7, lines. mentioned, as standards high-performance liquid (HPLC) quality, studied individually combination. Cytotoxic observed might be due to presence rutin. Benzalkonium strongest growth suppression effect cells (IC50 8.10−7 M) 5.10−6 M). mixture stachydrine allowed synergistic all tested normal obtained. Anti-cancer remains under-investigated, so this research describes how major can exert significant dose dependent cytotoxicity.